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Recombinant Protein G

Protein G

Recombinant Protein G

The gene we selected to use for the production of Protein G originated from Streptococcus Strain G148, and was cloned and expressed in our proprietary strain of Escherichia coli. The regions of the gene corresponding to the albumin binding domains, as well as the cell wall and cell membrane binding domains, have been genetically deleted, however the Fc binding domain is still present. Our recombinant Protein G contains 196 amino acid residues and has a predicted molecular mass of approximately 22.5 kDa, but migrates with an apparent molecular mass of about 30 kDa in SDS-PAGE.

G5: 2ug of recombinant Protein G resolved by SDS-PAGE reducing condition, showing a single band of 22.5 kDa.

Protein G

Protein Description

Recombinant Streptococcus Protein G is produced in E. coli and is a non-glycosylated polypeptide chain containing 196 amino acids. Streptococcus Protein G is proprietarily produced in E. coli and purified via chromatographic techniques. The product obtained is consistent in quality and yield because our proprietary bacterial platform is engineered to be deficient of major proteolytic activities. Protein G binds through a non-immune mechanism specifically to the Fc region of IgG and to all the human subclasses. It also has a strong affinity to monoclonal mouse IgG1 and rat IgG.

25mg Package

Recombinant Protein G

Formulation: Lyophilized Salt-free powder
Product#: G100L20

$99.00 USD each

75mg Package

Recombinant Protein G

Formulation: Lyophilized Salt-free powder
Product#: G100L50

$199.00 USD each

Bulk Size

Recombinant Protein L

Formulation: Salt-free powder
grams and larger lots available.

Available upon request

Source

Escherichia coli

Storage

Store at 4°C if entire vial will be used within 5 weeks. Store, frozen at -20°C for longer periods of time. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Purity and Formulation

≥95% by SDS-Page and HPLC analysis. Lyophilized with no additives, salt free.

Applications

MicroProtein’s material is furnished for laboratory or bioprocessing purposes. The product may not be used as a drug for humans or animals.

For Advocates of Personal Health

Organic Ingredients

Vegan Friendly

Non CMO Ingredients

Naturally Gluten Free

Naturally Diary Free

Escherichia coli

Store at 4°C if entire vial will be used within 5 weeks. Store, frozen at -20°C for longer periods of time. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

≥95% by SDS-Page and HPLC analyses. Lyophilized with no additive.

MicroProtein’s material is furnished for laboratory or bioprocessing purposes. The product may not be used as a drug for humans or animals.

25mg Package

Recombinant Protein G

Formulation: Lyophilized Salt-free powder
Product#: G100L20

$99.00 USD each

75mg Package

Recombinant Protein G

Formulation: Lyophilized Salt-free powder
Product#: G100L50

$199.00 USD each

Bulk Size

Recombinant Protein L

Formulation: Salt-free powder
grams and larger lots available.

Available upon request

Our Clients

We Serve All Over The World

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