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Cell Line Development

CMO

Microbial Cell Banking

MPT Biologics manufactures and cryo-stores Working and Master Cell Banks to support current and future upstream and downstream requirements. Microbial Cell Banks are cryo-stored in the vapor phase of liquid nitrogen freezers. MPT manages and coordinates all required testing per ICH and FDA guidelines.

MPT has experience with modular MPTxpress of E.coli. MPTxpress processes are demonstrated in process development and scaled-up for GMP in our facility.

 

MPTxpress Process Development Services and Capabilities

 

MPT routinely performs upstream and downstream development and optimization studies for our clients, is a critical step towards efficient design of GMP-compliant manufacturing processes.

Protein Purification and Bulk Fill

 

MPT has experience with a wide range of protein purification capabilities to meet client’s GMP production needs. Protein purification areas at MPT are segregated and customized with equipment to meet the requirements of client’s process. Protein purification personnel at MPT are experts with protein refolding, multiple chromatography techniques, tangential flow filtration, lyophilization, and bulk drug substance filling.

Few samples of CRO and CMO projects performed at MicroProtein Technologies Inc.

G1: SDS-PAGE Analysis of Biologic Drug Substance SB1 (sdAbs/Nanobodies).
Lane 1, M.W marker; Lane 2, SB1 5ug reducing condition; Lane 3, SB1 5ug non-reducing condition.
GMP grade Biologic Drug Substance SB1 manufactured at MicroProtein Technologies.

G2: SDS-PAGE Analysis of Biologic Drug Substance KB1 (Enzyme drug).
Lane 1, MW markers; Lane 2, 0.5ug; Lane 3, 1ug; Lane 4, 2ug reducing condition.
GMP grade Biologic Drug Substance KB1 manufactured at MicroProtein Technologies.

G3: 2ug of GMP-grade recombinant human FGF basic resolved by SDS-PAGE reducing conditions, showing a single band at 17 kDa.

G4: 2ug of recombinant human IL-2 resolved by SDS-PAGE reducing condition, showing a single band at 15.5 kDa.

G5: 2ug of recombinant Protein G resolved by SDS-PAGE reducing condition, showing a single band of 22.5 kDa.

G6: 2ug of recombinant Protein A resolved by SDS-PAGE reducing condition, showing a single band of 35 kDa.